Sensitive DNA detection for microelectrodes using HCR and DNAzyme electrocatalytic wires. Group I. Willner.
The DNA analyte-induced hybridization chain reaction (HCR) on electrode surfaces and the generation of hemin/G-quadruplex DNAzyme electrocatalytic wires, Figure 1 was used for the amplified and multiplexed electrochemical detection of DNA, Figure 1. Faradaic impedance spectroscopy, chronocoulometry and electrocatalyzed reduction of H2O2 using hemin/G-quadruplex DNAzyme labels were used as transduction means for the sensing events. Using several electrodes, multiplexed analysis of several DNA targets was demonstrated. The sensing platforms provided can be adapted for multiplexed sensing on chip arrays.